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Pouring Sake

Fermentation & biotechnology

Fermented food is nutritional food that has many benefits for health and has longer shelf-life.

Research publication highlights

Bacterial cellulose composite with antibacterial properties

Process efficiency improvement

By employing co-cultivation technique on Komagataeibacter xylinum and Lactococcus lactis subsp. lactis, bacterial cellulose (BC)/nisin films with improved antibacterial activity and mechanical properties were successfully produced. The findings demonstrated that increased nisin production is associated with an upregulation of gene expression. Furthermore, results from Scanning electronic microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and Thermogravimetric analysis (TG) confirmed the integration of nisin within BC. While being biocompatible with human cells, the BC/nisin composites exhibited antimicrobial activity. Moreover, mechanical property analyses showed a noticeable improvement in Young's modulustensile strength, and elongation at break by 161, 271, and 195 %, respectively. Additionally, the nisin content in fermentation broth was improved by 170 % after co-culture, accompanied by an 8 % increase in pH as well as 10 % decrease in lactate concentration. Real-time reverse transcription PCR analysis revealed an upregulation of 11 nisin-related genes after co-cultivation, with the highest increase in nisA (5.76-fold). To our knowledge, this is the first study which demonstrates that an increase in secondary metabolites after co-culturing is modulated by gene expression. This research offers a cost-effective approach for BC composite production and presents a technique to enhance metabolite concentration through the regulation of relevant genes.


Check out the article at International Journal of Biological Macromolecules 258 (2024) 128977

Exopolysaccharide production using PCS

Fermentation enhancement strategy 

Repeated-batch fermentation with fungal mycelia immobilized in plastic composite support (PCS) eliminates the lag phase during fermentation and improves metabolite productivity. The strategy is implemented herein, and a novel modified PCS is developed to enhance exopolysaccharide (EPS) production from the medicinal fungus Cordyceps militaris. A modified PCS (SYE + PCS) was made by compositing polypropylene (PP) with a nutrient mixture containing soybean hull, peptone, yeast extract, and minerals (SYE+). The use of SYE + PCS has consistent cell productivity throughout the multiple fermentation cycles, which resulted in a more higher cell productivity after second batch compared to unmodified PCS. The cell grown on SYE + PCS also generates a higher yield of EPS (3.36, 6.93, and 5.72 g/L in the first, second, and third fermentation cycles, respectively) up to three-fold higher than the cell immobilized on unmodified PCS. It is also worth noting that the EPS from mycelium grown on SYE + PCS contains up to 2.3-fold higher cordycepin than those on unmodified PCS. The presence of nutrients in SYE + PCS also affects the hydrophobicity and surface roughness of the PC, improving mycelial cell adhesion. This study also provides a preliminary antioxidant activity assessment of EPS from immobilized C. militaris grown with SYE + PCS.


Check out the article at International Journal of Biological Macromolecules 250 (2023) 126267

Other related publications ...

Current progress on the production, modification, and applications of
bacterial cellulose

Critical Reviews in Biotechnology 40 (2020) 397-414

Enhanced production of bacterial cellulose by Komactobacter intermedius using statistical modeling

Cellulose 27 (2020) 2497-2509

Atmospheric cold plasma-assisted pineapple peel waste hydrolysate detoxification for the production of bacterial cellulose

International Journal of Biological Macromolecules 175 (2021) 526–534

Using Formic Acid to Promote Bacterial Cellulose Production and Analysis of Its Material Properties for Food Packaging Applications

Fermentation 8 (2022) 608

Evaluation of porous bacterial cellulose produced from foam templating
with different additives and its application in 3D cell culture

International Journal of Biological Macromolecules 234 (2023) 123680

Synthesis of Novel Bacterial Cellulose Based Silver-Metal Organic
Frameworks (BC@Ag-MOF) as Antibacterial Wound Healing

Fine Chemical Engineering 4 (2023) 193-202

Enhanced exopolysaccharide production of Cordyceps militaris via mycelial
cell immobilization on plastic composite support in repeated-batch fermentation

International Journal of Biological Macromolecules 250 (2023) 126267

Production of bacterial cellulose (BC)/nisin composite with enhanced
antibacterial and mechanical properties through co-cultivation of Komagataeibacter xylinum and Lactococcus lactis subsp. lactis

International Journal of Biological Macromolecules 258 (2024) 128977

Improvement of extracellular polysaccharides production from Cordyceps militaris immobilized alginate beads in repeated-batch fermentation

LWT - Food Science and Technology 193 (2024) 115752

Agar-altered foaming bacterial cellulose with carvacrol for active food packaging applications

Food Packaging and Shelf Life 42 (2024) 101269

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